TB

Tuberculosis, or TB, is an infectious disease which is mainly caused by Mycobacterium Tuberculosis.  TB majorly attack lungs but it does have its impact on other body systems as well, like bones, joints, central nervous system, the lymphatic system, the circulatory system, the gastrointestinal system, etc. Tuberculosis is an infectious disease which is transmitted from an infected person to another. The mode of transmission is through the air, as when a person with TB coughs, sneezes or even talks, droplets transfers to the air which is later inhaled by the other person. When this infected air reaches the lungs to the healthy person, it spread its infection, and hence another person also catches tuberculosis. Person with weak immune system are likely to get tuberculosis easily, as  “the immunity or the defense wall” against infection is not strong enough to safeguard against the infection. Prolonged exposure to TB infected person maximizes the risk of getting TB.

Signs and Symptoms: +

The typical signs and symptoms of tuberculosis are as follows.

  • Persistent coughing that lasts more than 3 weeks
  • Unexplained weight loss
  • Fever
  • Coughing up blood
  • Chest pain
  • Pain while breathing
  • Loss of appetite
  • Night sweats
  • Weakness or fatigue
  • Breathing difficulty
  • Wheezing

General diagnostic tests suggested:+

Diagnosis of active tuberculosis based on signs and symptoms is not only difficult, but its results are also not accurate. Hence proper diagnosis is suggested so that the exact condition of the TB infection can be detected out that it is an active tuberculosis state or it is in a latent state. Following are the tests generally performed to diagnose tuberculosis.

  • Biopsy of the affected tissue
  • Chest X-ray
  • Bronchoscopy
  • Chest CT Scan
  • Thoracentesis
  • Tuberculin skin test
  • Sputum examination and cultures
  • Interferon-gamma blood test
  • Nucleic acid amplification test
  • Adenosine deaminase test
  • Mantoux tuberculin skin test

Test available at Oncquest:+

Following are the tuberculosis test carried out at Oncquest along with the details of the test such as test code, cost involved, the technique used, sample required, special instructions to be kept in mind while giving a sample, run days at section and reported dates.
1.  Test:  T. B (TUBERCULOSIS) test which includes Gamma Interferon / T.B GOLD and T.B Feron (In – Tube Test)
Test Code: 0754 MS
Cost Involved: Rs. 3,300
The technique used: Enzyme Immunoassay
Sample required: 2 – 3 ml. Whole blood collected & submitted in a set of 3 special QFT GOLD – IT tubes available from Oncquest Laboratories Ltd. Shake the tubes vigorously at least 10 times to ensure thorough mixing. DO NOT CENTRIFUGE. Send immediately at room temperature to reach the laboratory within 16 hrs of sample collection. Or incubate the tubes immediately at 37°C for 16 – 24 hours.  Send refrigerated within 72 hours.
Special Instruction: 12-14 hrs (Overnight) fasting is preferred.
Run days at section: Monday, Wednesday & Friday.
Reported on: 6th Working Day if received before 1400 Hrs.

2. Test:  T. B (TUBERCULOSIS) test which includes T. B PCR (Miscellaneous Specimens)
Test Code: S0756 MS
Cost Involved: Rs. 1,700
The technique used: PCR
Sample required: Pus/Body fluids (Pleural/Ascitic/Pericardial/Synovial/Ocular) Aspirates/ Semen /BAL /Bronchial washings: Submit as much as possible 3 – 5 ml.(1 ml. Minimum) in a sterile, plastic, screw capped container. Send Refrigerated. DO NOT FREEZE. Specify type of specimen on container and TRF.
Run days at section: Every day.
Reported on: 4th Working Day if received before 1200 Hrs.

3. Test:  T. B (TUBERCULOSIS) test which includes T.B Real Xpert T. B Real Time PCR (Miscellaneous Specimens)
Test Code: S0757 MS
Cost Involved: Rs. 2,800
The technique used: Real Time PCR
Sample required: Gastric Lavage: Patient should be fasting for previous 12 hours.Transport 5 – 10 ml. of gastric lavage specimen within 4 hours of collection. Send Refrigerated. DO NOT FREEZE. Specify type of specimen on container and TRF.
Run days at section: Every day.
Reported on: 4th Working Day if received before 1200 Hrs.

 

4. Test:  MTB Transcription Mediated Amplification (TMA)
Test Code: 0231OT
Cost Involved: Rs. 3,000
The technique used: Transcription Mediated Amplification (TMA)
Sample required: Sputum/Body fluids or aspirates/Urine: First morning Sputum or 1 ml. or as much as practically possible body fluids/aspirates or 100 ml. first morning mid-stream urine.
Run days at section: Alternate Day.
Reported on: 3 Working Days.

5. Test:  MTB Drug Resistance – 1 Line of Drugs
Test Code: 0232OT
Cost Involved: Rs. 5,000
The technique used: PCR & Linear Array Hybridization
Sample required: Bone Marrow/Menstrual blood: Bone marrow is collected in EDTA tube. Menstrual blood of first day of menstrual cycle to be sent in a sterile container.
Run days at section: Alternate Day.
Reported on: 17-20 Working Days.

6. Test:  MTB Drug Resistance – 2 Line of Drugs
Test Code: 0233OT
Cost Involved: Rs. 7,000
The technique used: PCR & Linear Array Hybridization
Sample required: CSF: Optimally 2 ml CSF should be collected in a sterile disposable container. Minimum 0.5 ml of
Run days at section: Alternate Day.
Reported on: 17-20 Working Days.

7. Test:  MOTT Differentiation (Mycobacterium other than TuberculosisDifferentiation)
Test Code: G23S05T06
Cost Involved: Rs. 5,000
The technique used: PCR & Linear Array Hybridization
Sample required: Fresh Tissue/Bone Tissue Biopsy: A portion of tissue suspected to be infected with Mycobacterium species should be taken in normal saline in sterile container. Culture: 1-2ml liquid TB culture broth collected in a sterile container. Whole blood samples are not accepted for the test. Specimens must be collected in sterile containers. Transport samples at frozen condition within 24 hrs of collection.
Run days at section: Alternate Day.

Reported on: 17-20 Working Days.