Cancer HOT SPOT Panel – 50 Genes by NGS
- Allows translational and disease researchers to fast track oncology research by surveying hotspot regions of 50 oncogenes and tumor suppressor genes with wide coverage of many genes including KRAS, BRAF and EGFR genes.
- Breakthrough technology with improved primer design, containing 207 primer pairs in a single tube and requires as little as 10ng of DNA, enabling researchers to sequence challenging samples such as formalin-fixed and paraffin-embedded (FFPE) tissue (, which are typically available only in small amounts and often exhibit variable quality).
- Help in targeted therapy, reducing costs and improving the outcome of therapy
- Very significant in cases where the patient has failed 1st / 2nd line of therapy or there has been no actionable mutation from the preliminary testing
- A comprehensive information of the actionable mutations at the outset can help in timely treatment
Helpful when trying to enrol the patient for clinical trials
Next Generation Sequencing (NGS)
A highly sensitive technology, which can detect mutations in samples having as low as 5% tumor cells. It can be multiplexed that is many genes can be targeted at the same time, thus detecting various mutations in the same assay. Each DNA nucleotide is read several times (100-1000 times) which adds to the specificity of the assay. This platform provides accurate, reliable data and excellent coverage.
Advantages of NGS
- All at Once Approach for Cancer Diagnostics
- NGS captures a broader spectrum of mutations
- Comprehensive view of the most “actionable mutations” in solid tumors with greater in depth coverage,
thus leading to enhanced sensitivity
- The increased sensitivity of NGS allows detection of mosaic mutations
- As low as 5% of mutant DNA can be detected, hence very low amount of DNA (~10 -50ng) as specimen is required
- Faster TAT as compared to sequential gene testing using conventional methods